- A graduated container of 100 ml.
- Counting solution: sodium citrate (3.4%) treated with formaldehyde.
- 1 ml automatic micropipette with single-use tips.
- Bürker Chamber.
Make a 100 ml solution:
- 1 ml of pure semen.
- 99 ml of counting solution.
- Shake the bottle and place a drop on the counting chamber.
Count the sperm cells contained in 40 small squares of two grids. Compute only those sperm cells with the heads inside the square, in contact with the top line, the left side and two corners. The resultant number will be the average of the 4 series of 40 squares.
Calculation of sperm number in Bürker chamber (use concentration)
A x 107 x V A x V
No. of doses (N) = —— = —-
3 x 10 ^ 9 300
V = Volume of the ejaculate in cc
A = Number of sperm counted in 40 squares (0.01 mm3)
Total number of sperm in the ejaculate:
in 1 mm3 = A x 100
in 1 cc = (A x 100) x 1,000
in pure semen (dilution (A x 100) x 1,000 x 100 = A x 1072520-camara-burker-covers011
Total spermatozoa in the ejaculate = A x 107 x V
Amount of diluent to be added (seminal doses of 100 cc):
N x 100 = Total Volume (Thinner + Ejaculate)
N x 100 – V = Volume of Diluent to be added
Recommended dilution 1/10 – 1/25